Higher serum Sirtuin 1 levels and GA heterozygote of SIRT1 gene polymorphism rs10823108 serve as independent risk factor for diabetic nephropathy in women

Abstract

IntroductionDiabetic nephropathy (DN) has been one of the most common conditions in patients with type 2 diabetes mellitus (T2DM) and risk of continuous increasing rate of morbidity and mortality worldwide. This study aimed to evaluate the vulnerability of SIRT1 gene polymorphisms with its serum levels in susceptibility to DN and T2DM. MethodsA total of 180 individuals including 60 patients with DN (43 men and 17 women), 60 with T2DM (23 men and 37 women) and 60 healthy controls (24 men and 36 women) were enrolled in age range of 40 to 70 years irrespective of their gender from South Indian population. Venous blood samples was collected from each study participant and used for genotype and allele frequency distribution analysis of rs3818292 and rs10823108 polymorphisms of SIRT1 gene using PCR-RFLP, and estimation of serum Sirtuin 1 levels. ResultsGenotype and allele frequency distribution analysis of rs3818292 polymorphism didn't show significant difference in patients with DN than T2DM. However, rs10823108 polymorphism showed significantly increased (35%) risk of GA genotype in DN women than T2DM (p < 0.05). Although serum Sirtuin 1 levels were significantly higher in both DN and T2DM patients compared to control individuals, no genotype association was observed with both the polymorphisms (p > 0.05). ConclusionGA genotype of rs10823108 polymorphism of SIRT1 gene induces 35% increased risk of DN in women compared to T2DM. Serum Sirtuin 1 levels are increased in DN and T2DM patients than control individuals, no significant association was observed between genotype frequency distribution and Sirtuin 1 levels.