Abstract
The use of single crystal support films to produce high contrast images of phase objects has been discussed by Hines and Howie (1). When a phase object such as a biological molecule or a latex sphere is located on top of a strongly diffracting crystal support, the crystal support acts like a phase plate. Dark field, shadow or phase contrast images may be formed depending upon the exact crystal diffraction conditions and thickness. An objective aperture is used to exclude the diffracted beams so that the image is formed only with the bright field beam and the electrons scattered by the object. For simplicity in analysis, 0.11 micron diameter latex spheres are used as objects and are supported on graphite crystals about 0.1 microns thick. The graphite crystal preparation is described elsewhere (2). The latex sphere suspension is sprayed onto the graphite with a glass nebulizer after the graphite has been made hydrophilic by subjecting it to a glow discharge.
Published Version
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