Abstract
SummaryA 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.
Highlights
Human embryonic, induced pluripotent, and adult stem cells are invaluable tools for drug discovery, human toxicology, and studies on human development
Cells are encapsulated in a 3D matrix atop a PillarChip, which is submerged into a WellChip for on-chip culture (Figure 1)
On-chip 3D cultures were rapidly dried for image processing and analysis; for samples labeled with fluorescent livecell stains, this resulted in cell lysis. It was reasoned this cell lysis could be prevented with a lyoprotectant, and addition of 50 mM trehalose to the Dulbecco’s phosphate-buffered saline (DPBS) wash solution prior to drying preserved cytoplasmic staining of calcein, enabling the simplified imaging approach (Figure S1A)
Summary
A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells
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