Abstract
Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 102 CoViD-19 and non-CoViD-19 patients. The SPRi assay simultaneously measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19. This new high throughput method can be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 and its mutants in vaccination programs.•Surface Plasmon Resonance imaging is an unprecedented technology for high throughput screening of antibody profiling of CoViD19 patients.•Fingerprinting of isotypes IgM, IgG and IgA can be performed for 384 patients in one run.•An affinity maturation effect was shown for patients recovering from CoViD19.
Highlights
As indicated in the original condensed manuscript [Schasfoort, R.B.M., et al, 2021], a combined measurement of the anti-RBD IgM, IgG and IgA specific levels of CoViD-19 patients is possible in a single experiment using Surface Plasmon Resonance imaging (SPRi)
Method name: Clinical antibody detection generated by SARS-CoV-2 with high throughput Surface Plasmon Resonance imaging Keywords: Corona, Infectious diseases, Antibody isotypes, Affinity, Off-rate, High throughput, Biosensor Article history: Received 17 March 2021; Accepted June 2021; Available online June 2021
One can find details of features, benefits and tricks. Both SPR imagers can be applied for detection of the composition and affinity of the serum isotype antibodies of CoViD19 patients.The specific anti-RBD antibody concentration cannot be determined because of the polyclonality of the serum that generates antibodies to all kinds of epitopes of SARS-CoV2
Summary
High throughput surface plasmon resonance imaging method for clinical detection of presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 This new high throughput method can be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 and its mutants in vaccination programs. Method name: Clinical antibody detection generated by SARS-CoV-2 with high throughput Surface Plasmon Resonance imaging Keywords: Corona, Infectious diseases, Antibody isotypes, Affinity, Off-rate, High throughput, Biosensor Article history: Received 17 March 2021; Accepted June 2021; Available online June 2021.
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