High-throughput sequencing reveals genes associated with high-temperature stress tolerance in sugarcane.

Abstract

Sugarcane (Saccharumspp.) is a major sugar crop grown in tropical and sub-tropical areas throughout the world which is vulnerable to high temperature stress due to climate change. In this present study, we have generated a transcriptome profile of sugarcane variety Co 99004 exposed to high-temperature stress (47°C). The Illumina Nexseq2500 platform yielded a total of 39.28 and 13.44 million reads, corresponding to 3.9 and 1.3 gigabase pair (Gb) of the processed reads for control and high-temperature-stressed samples, respectively. Initially, the reads were de novo assembled into 118,017 unigenes with an average length of 780bp. The longest sequence in the assembly was 21kb. Further, these transcripts were BLASTed against GO, KEGG and COG databases to identify the novel genes/transcripts expressed due to elevated temperature conditions. The different expression analysis showed 1137 transcripts which were up-regulated during heat temperature stress when compared to control conditions. Analysis of relative gene expression showed phytepsin, ferredoxin-dependent glutamate synthase, and stress protein DDR-48 threefold increased expression during heat stress. These findings reveal novel targets for subsequent research on the genomics genetic manipulation and molecular mechanism of elevated stress tolerance in sugarcane.