High-Throughput PCR for DNA Part Generation.

Abstract

This protocol describes a high-throughput approach to PCR for the generation of over a thousand amplicons in parallel. Modern liquid handling robotics are used to accelerate reaction setup, miniaturize reaction volumes, and dramatically reduce reagent and consumable cost. Although the focus is on generating DNA parts for use in DNA assembly techniques, this methodology can be applied to any workflow where the parallel production of hundreds or thousands of PCR amplicons is required.