Abstract
Teresa Artuso, Bruce Griffin, Monica HT Wong and Frank Merante* Author Affiliations Seneca College, School of Biological Sciences and Applied Chemistry, Ontario, Canada Received: June 28, 2021 | Published: July 22, 2021 Corresponding author: Frank Merante, Seneca College, School of Biological Sciences and Applied Chemistry, Ontario, Canada DOI: 10.26717/BJSTR.2021.37.005993
Highlights
Pyruvate is a commonly occurring organic keto-acid found in various food-associated items and many biological fluids
A 96-well, high throughput, colorimetric chemical detection method based on 2,4-dinitrophenyl hydrazine (DNPH) reaction of the keto-acid to determine the concentration of pyruvate in human sweat samples is presented
Pyruvate quantification in onions is of central importance for the selection of breeding stock that imparts lower pungency and enhanced sweetness, which correlate to consumer preference [6,7,8]
Summary
Pyruvate is a commonly occurring organic keto-acid found in various food-associated items and many biological fluids. A 96-well, high throughput, colorimetric chemical detection method based on 2,4-dinitrophenyl hydrazine (DNPH) reaction of the keto-acid to determine the concentration of pyruvate in human sweat samples is presented.
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