High tetragonula sp honey addition reduce cell proliferation on fibroblast preputium culture

Abstract

Induced pluripotent stem cells can be engineered by protein transduction from fibroblast cell, that has been obtained from skin tissue. To increase the protein transduction, needs optimization culture medium. Fetal Bovine Serum was replaced by honey from Tetragonola sp. to reduce the protease and increased the protein transduction efficiency. The purposed of this preliminary research were to determine the comparison between DMEM media-free serum addition of Tetragonula sp honey on fibroblast preputium cell proliferation. The design of this study used true experimental method. The sample of preputium skin was taken from a healthy child <13 years old. The fibroblast cells isolated from tissue explants, were cultured by variations (0,1%, 1%, 5%) honey concentration, then measured using MTT assay. Fibroblast cell culturing in medium supplemented with 5% Tetragonula sp honey showed significant difference of less proliferation than standard medium with fetal bovine serum (p = 0.000). Medium supplement with 0,1% Tetragonula sp honey was significant difference of higher proliferation compare to 1% (p = 1.000) and 5% (p = 0.000) treatment medium, but still cannot overleap standar medium with fetal bovine serum. Abundant sugar (dominant in honey) in the culture medium can inhibit fibroblast cells growth. The search for a safe and effective Fetal Bovine Serum (FBS) substitution in fibroblast preputium cell cultures must continue to be developed.