Abstract
Polyhydroxyalkanoates (PHAs) are intracellular storage polymers that enhance bacterial resistance in environments. While the role of PHAs regulation in thermophiles under high-temperature stimulation is understudied, this work investigates Aeribacillus pallidus BK1, a thermophile with heat resistance up to 155 °C. Our results showed that A. pallidus’s PHAs yield was 1.45 g/L. After 90 °C and 121 °C stimulations, the PHAs yield doubled to 3.33 g/L. The PHAs ratios increased from 35.63 % (60 °C) to 75.46 % (90 °C) and 77.15 % (121 °C). RNA-seq analysis revealed a common strategy of activating glucose transporters to enhance glucose uptake at both temperatures. At 90 °C, A. pallidus BK1 prioritized PHAs accumulation over the TCA cycle. At 121 °C, PHAs production was further enhanced by upregulating monomer polymerization and downregulating acetyl-CoA carboxylase expression. These findings offered valuable insights into the high-temperature defense mechanisms of thermophiles and suggested that A. pallidus BK1 holds promise as a bio-production platform for PHAs production under thermal stimulation.
Published Version
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