High-speed precise cell patterning by pulsed electrohydrodynamic jet printing

A V Makaev,V Ya Shur,I V Zubarev,V R Karpov,O S El’Kina,E A Mingaliev

doi:10.1088/1757-899x/256/1/012013

A V Makaev, V Ya Shur + Show 4 more

Open Access

https://doi.org/10.1088/1757-899x/256/1/012013

Copy DOI

Abstract

The generation of micro-droplets of nutrient medium with living cells by pulsed electrohydrodynamic printing has been studied. In-situ visualization by high-speed camera made it possible to measure the characteristic times of droplet generation process and to determine the optimal printing parameters. Maximal frequency of stable generation was achieved at 700 Hz. This technique was applied successfully for drop-on-demand printing of culture medium with live HeLa cells and yeasts.

Highlights

Electrohydrodynamic (EHD) printing is a method of generating a droplet or a continuous jet by the application of an electric field to drop meniscus [1]
EHD printing is driven by a strong electric field generated by a voltage between the nozzle with charged liquid and a grounded electrode [3, 4]
We have demonstrated essential improvement of the main characteristics of EHD printing method using bias voltage with opposite sign to pulse voltage for minimizing the oscillation amplitude and the damping time

Summary

Introduction

Electrohydrodynamic (EHD) printing is a method of generating a droplet or a continuous jet by the application of an electric field to drop meniscus [1]. 1. Introduction Electrohydrodynamic (EHD) printing is a method of generating a droplet or a continuous jet by the application of an electric field to drop meniscus [1].

Results

Conclusion