Abstract
For the separation and determination of the closely related, cyclic undecapeptides cyclosporin A, B, C and D in samples from fermentations, a high-speed liquid chromatographic method with conventional apparatus has been developed, based on a separation with 3-μm Nucleosil C 8 column and acetonitrile—water—phosphoric acid (700:300:0.1) as eluent at 70°C. The analysis time of about 3.5 min, the use of a sampler for 195 vials and a laboratory data system offer a high degree of automation and analytical economy. The coefficient of variation is about 0.8–1.5% ( n = 10). The rather low plate counts for the cyclosporins are due to the structures and high molecular weights of these substances.
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