Abstract
When high-speed liquid chromatography is used to obtain pure material for subsequent investigation, it can correctly be called preparative, regardless of the sample weights employed. In this paper, the requirements for scaling up from a small-diameter analytical column to larger-diameter preparative columns are presented. An example of the use of high-speed preparative liquid chromatography to isolate and identify the components of a complex mixture is presented. General requirements of sample size for subsequent investigation are discussed.
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