Abstract
Septins are a family of GTP-binding cytoskeleton proteins expressed in many solid tumors. Septin 9 (SEPT9) in particular was found overexpressed in diverse carcinomas. Herein, we studied the expression of SEPT9 isoform 1 protein (SEPT9_i1) in human prostate cancer specimens. We utilized immunohistochemical staining to study the expression of SEPT9_i1 protein. Staining level was analyzed in association with clinical characteristics and the pathological Gleason grade and score. Fifty human prostate cancer specimens (42 primary tumors and 8 metastatic lesions) were stained by SEPT9_i1 antibody and analyzed. SEPT9_i1 protein was expressed in prostate cancer cells but absent in normal epithelial cells. The intensity of staining was correlated proportionally to pretreatment prostate-specific antigen (PSA) blood levels and Gleason score (P < 0.05). SEPT9_i1 was highly expressed in all metastatic lesions. A significant assocation between SEPT9_i1 expression and high Gleason score on multivariate linear regression analysis was found. We conclude that SEPT9_i1 is expressed in high-grade prostate tumors suggesting it has a significant role in prostate tumorigenesis and that it could serve as a molecular marker for prostate tumor progression.
Highlights
Prostate cancer is second to lung cancer in incidence worldwide and is the second most common cancer, and one of the leading causes of cancer deaths among Western men [1]
Archival material prior to the year 2000 was approved for use by the institutional review board of the Hebrew University-Hadassah Medical Center, with a waiver of informed consent, in accordance with the State of Israel Law of Genetic Information, 2002
All study specimens were prior to year 2000 and all were de-identified. This was a retrospective study on a series of 50 paraffin-embedded prostate cancer samples: 38 from radical prostatectomy, 6 from radical cystoprostatectomy and 6 from transurethral resection of prostate (TUR-P)
Summary
Prostate cancer is second to lung cancer in incidence worldwide and is the second most common cancer, and one of the leading causes of cancer deaths among Western men [1]. The primary risk factors for prostate cancer are age and family history. Prostate cancer becomes more common with advancing age affecting men aged 50 or more [2]. Prostate adenocarcinoma metastasizes mainly to the bones and less commonly to lymph nodes, and may locally advance to invade neighboring organs. Current treatments include hormonal therapy, immunotherapy and chemotherapy, yet there is no curative treatment for metastatic prostate cancer [3, 4]. Novel strategies for treatment of prostate cancer and identification and characterization of new molecular targets such as interleukin-6 are essential [5]
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