Abstract 3995: Differential expression of CD10 immunohistochemistry in stroma and epithelium of prostatic adenocarcinoma

Abstract

Abstract Introduction: CD10 is a transmembrane metallo-endopeptidase involved in cleavage and inactivation of certain peptide hormones important for signal transduction. CD10 is strongly expressed by normal prostatic luminal epithelial cells. Immunohistochemical (IHC) studies have shown expression of CD10 in prostatic adenocarcinoma. (PCa). Loss of CD10 expression is a common, early event in human prostate cancer (PCa); however, CD10 positive cancer cells frequently appear in lymph node metastasis. Only a few studies are available on the expression of CD10 in the prostatic stromal and cancer cells. We investigated the IHC expression of CD10 in epithelium and stroma of PCa using TMAs. Material and Methods: Prostate tissue from 72 radical prostatectomies was used to construct two TMAs; 35 case of Gleason score (GS) 6(3+3) and 37 cases of high grade (HG) prostate cancer (GS 8, 9, 10) were included. Each case was represented by three, 1mm cores for a total of 105 cores for the GS6 and 111 cores for HG prostate carcinomas. TMAs were stained with H&E and IHC was performed for CD10. Digital images of the TMA slides were scanned using the T3 ScanScope by Aperio Technologies. Epithelial and stromal expression of CD10 was scored as negative or positive. Results: CD10 was expressed in epithelium and stroma of PCa. CD10 had heterogeneous expression within individual patients in both epithelium and stroma. This heterogeneity was not significantly different between GS6 and HG prostate cancer. CD10 was differentially expressed in epithelium of GS6 and HG prostate cancer. HG prostate cancer showed significantly more epithelial staining with CD10 (p=0.001) and less stromal staining with CD10 (p=0.003) compared to GS6. Conclusion: GS6 and HG prostate cancer demonstrate a reverse relationship between CD10 expression in the epithelium and the stroma. Epithelial CD10 expression is an unfavorable risk factor in prostate cancer. Prostate tumors expressing high levels of epithelial CD10 correlate with a poor disease outcome. CD10 may be important in the biology of the tumor stromal microenvironment and its contribution to tumor progression requires further investigation. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3995.