Abstract
In this study, a high sensitive and selective hydrogen peroxide (H2O2) sensor was successfully constructed with Pt-Au bimetallic nanoparticles (Pt-Au NPs)/reduced graphene sheets (rGSs) hybrid films. Various molar ratios of Au to Pt and different electrodeposition conditions were evaluated to control the morphology and electrocatalytic activity of the Pt-Au bimetallic nanoparticles. Upon optimal conditions, wide linear ranges from 1 µM to 1.78 mM and 1.78 mM to 16.8 mM were obtained, with a detection limit as low as 0.31 µM. Besides, due to the synergetic effects of the bimetallic NPs and rGSs, the amperometric H2O2 sensor could operate at a low potential of 0 V. Under this potential, not only common anodic interferences induced from ascorbic acid, uric acid and dopamine, but also the cathodic interference induced from endogenous O2 could be effectively avoided. Furthermore, with rat pheochromocytoma cells (PC 12) as model, the proposed sensor had been successfully used in the detection of H2O2 released from the cancer cells. This method with wide linear ranges and excellent selectivity can provide a promising alternative for H2O2 monitoring in vivo in the fields of physiology, pathology and diagnosis.
Highlights
H2O2 is an indicator of oxidative stress [1], which is closely related to the physiological and pathological events such as aging, cancer, ischemia/reperfusion injury, traumatic brain injury, impaired learning and memory functions, and so on [2,3]
The higher sensitivity and wider linearity of this H2O2 sensor could be attributed to the addition of Au, which could effectively improve the electrocatalytic activity of Pt, especially the poisoning by adsorbed intermediates due to structure-induced promotional effects [22]
The results suggested a good reproducibility of the proposed H2O2 sensor
Summary
H2O2 is an indicator of oxidative stress [1], which is closely related to the physiological and pathological events such as aging, cancer, ischemia/reperfusion injury, traumatic brain injury, impaired learning and memory functions, and so on [2,3]. Tsai et al [14] fabricated electrochemical sensor based on the Au-Ag alloy nanoparticles, which exhibited high sensitivity and good selectivity for H2O2. Bimetallic nanoparticles such as Pt/Ag and Pt/Cu [16], Pt/Pd [17], Pd/Cu [18], Rh/Pd [19] and Ru/Rh [20] were applied for constructing an electrochemical platform for determination of H2O2, and most of them possessed great potential. The proposed H2O2 sensor, possessing high sensitivity, excellent selectivity, good reproducibility and long-term stability, had been proven to be promising for the practical application in the real-time monitoring of H2O2 in vivo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
