Abstract
We report on the use of high resolution Raman spectroscopy mapping combined with a micro-engineered stem cell platform. This technique obtains quantitative information about the concentration of individual intracellular molecules such as proteins, lipids, and other metabolites, while tightly controlling cell shape and adhesion. This new quantitative analysis will prove highly relevant for in vitro drug screening applications and regenerative medicine.
Highlights
Results and discussionDevelopment of cell micropattern arraysMicropatterned glass substrates containing fibronectin (FN)coated islands were fabricated using a modified literature protocol for micro-contract printing.[22]
We report on the use of high resolution Raman spectroscopy mapping combined with a micro-engineered stem cell platform
In order to investigate the findings obtained by Raman spectroscopy analysis, traditional immunohistochemical analysis was performed. Human mesenchymal stem cells (hMSCs) micropatterns stained against F-actin showed clear differences in cellular architecture between the different cell shapes
Summary
We report on the use of high resolution Raman spectroscopy mapping combined with a micro-engineered stem cell platform. This technique obtains quantitative information about the concentration of individual intracellular molecules such as proteins, lipids, and other metabolites, while tightly controlling cell shape and adhesion. This new quantitative analysis will prove highly relevant for in vitro drug screening applications and regenerative medicine
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