High-quality permanent draft genome sequence of the Lebeckia - nodulating Burkholderia dilworthii strain WSM3556(T).

Sofie E De Meyer,Ron Yates,Rui Tian,Victor Markowitz,John Howieson,Rekha Seshadri,Amrita Pati,Tanja Woyke,Nikos Kyrpides,Wayne Reeve,Natalia Ivanova

doi:10.1186/s40793-015-0048-3

Abstract

Burkholderia dilworthii strain WSM3556T is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective N2-fixing root nodule of Lebeckia ambigua collected near Grotto Bay Nature Reserve, in the Western Cape of South Africa, in October 2004. This plant persists in infertile and deep sandy soils with acidic pH, and is therefore an ideal candidate for a perennial based agriculture system in Western Australia. WSM3556T thus represents a potential inoculant quality strain for L. ambigua for which we describe the general features, together with genome sequence and annotation. The 7,679,067 bp high-quality permanent draft genome is arranged in 140 scaffolds of 141 contigs, contains 7,059 protein-coding genes and 64 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

Highlights

Over the last decade, agricultural scientists have sought to discover perennial legumes from a wide range of natural environments to develop new plants for grazing systems [1]
Lebeckia ambigua is proving well adapted to Western Australia [1] because in areas where it is naturally found
WSM3556T represents a potential inoculant quality strain for L. ambigua, which is being developed as a grazing legume adapted to infertile soils that receive 250–400 mm annual rainfall in southern Australia and is of special interest to the RNB chapter of the

Summary

Introduction

Agricultural scientists have sought to discover perennial legumes from a wide range of natural environments to develop new plants for grazing systems [1]. The isolation of bacteria from these nodules gave rise to a collection of 23 strains that were identified as Burkholderia [3]. Unlike most of the previously studied nodulating Burkholderia strains, this South African group appears to associate with papilionoid forage legumes, rather than Mimosa species.

Results

Conclusion