Abstract
ABSTRACTThe siderophilic, thermophilic Leptolyngbyaceae cyanobacterium JSC-12 was isolated from a microbial mat in an iron-depositing hot spring. Here, we report the high-quality draft genome sequence of JSC-12, which may help elucidate the mechanisms of resistance to extreme iron concentrations in siderophilic cyanobacteria and lead to new remediation biotechnologies.
Highlights
For genomic DNA isolation, JSC-12 was cultivated at 40°C under permanent white light (17 mE/m2/s) in 1-liter flasks containing 0.25 liter of DH medium supplemented with 0.04 mM FeCl3Á6H2O on a rotating shaker (18 rpm)
Prediction of biosynthetic gene clusters revealed that the JSC-12 genome lacks the pathway for synthesizing bacterioferritin, the classical iron storage protein
The JSC-12 genome can be found under the GenBank Nucleotide accession numbers CM001633.1, AJUB00000000.1, and GCF_000309945.1
Summary
For genomic DNA isolation, JSC-12 was cultivated at 40°C under permanent white light (17 mE/m2/s) in 1-liter flasks containing 0.25 liter of DH medium supplemented with 0.04 mM FeCl3Á6H2O on a rotating shaker (18 rpm). Prediction of biosynthetic gene clusters revealed that the JSC-12 genome lacks the pathway for synthesizing bacterioferritin, the classical iron storage protein.
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