Abstract
Pontibacillus yanchengensis Y32T is an aerobic, motile, Gram-positive, endospore-forming, and moderately halophilic bacterium isolated from a salt field. In this study, we describe the features of P. yanchengensis strain Y32T together with a comparison with other four Pontibacillus genomes. The 4,281,464 bp high-quality-draft genome of strain Y32T is arranged into 153 contigs containing 3,965 protein-coding genes and 77 RNA encoding genes. The genome of strain Y32T possesses many genes related to its halophilic character, flagellar assembly and chemotaxis to support its survival in a salt-rich environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0085-y) contains supplementary material, which is available to authorized users.
Highlights
Pontibacillus yanchengensis Y32T (= CGMCC 1.10680T = CCTCC AB209311T = NRRL B-59408T) was isolated from a salt field in Yancheng, China [1], and affiliated to the family Bacillaceae, order Bacillales, phylum Firmicutes [2, 3]
The genus contains six species, including Pontibacillus yanchengensis [1], Pontibacillus chungwhensis [4], Pontibacillus marinus [5], Pontibacillus halophilus [6], Pontibacillus litoralis [7], and Pontibacillus salicampi [8], which are isolated from a salt field, a solar saltern, a solar saltern, a sea urchin, a sea anemone, and a saltern soil, respectively
The phylogenetic tree based on the 16S rRNA gene sequences indicated that strain Y32T was clustered within a branch containing other species in the genus Pontibacillus (Fig. 1a)
Summary
The phylogenetic tree based on the 16S rRNA gene sequences indicated that strain Y32T was clustered within a branch containing other species in the genus Pontibacillus (Fig. 1a). P. yanchengensis Y32T is Gram-positive, rod-shaped (0.5–0.9 × 1.9–2.5 μm), motile with flagella (Fig. 2) and endospore-forming It can grow on Bacto marine broth 2216 (Difco) agar medium containing 3–20 % (w/v) NaCl and does not grow in the absence of NaCl [1]. KEGG pathway analysis of the five Pontibacillus genomes (see below) revealed that only strain Y32T had the key enzyme mannitol-1-phosphate 5-dehydrogenase (gene ID: N782_14920) which could potentially catalyze Dmannitol 1-phosphate to D-fructose 6-phosphate. This result was consistent with the phenotype. Transmembrane helices and signal peptides were predicted by the online bioinformatic tools TMHMM 2.0 [20, 21] and SignalP 4.1 [22], respectively
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