High production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from Bacillus subtilis KIBGE HAS

Abstract

Sugarcane bagasse is a cheap carbon source for endo-1,4-β-d-glucanase production as it is easily available as by-product from sugar industries. Fermentation conditions for endo-1,4-β-d-glucanase production by Bacillus subtilis KIBGE HAS were optimized by using un-treated sugarcane bagasse for induction of endo-1,4-β-d-glucanase and it was found that 2.0g% bagasse in fermentation medium induced maximum endo-1,4-β-d-glucanase production. It was also found that when sugarcane bagasse was supplemented with different carbon sources, the results showed that lactose, xylose, maltose and sucrose favored endo-1,4-β-d-glucanase production, whereas cellobiose and fructose inhibit enzyme production. Maximum endo-1,4-β-d-glucanase production was obtained at 40°C keeping the initial pH of the medium at 7.0 before sterilization. Maximum endo-1,4-β-d-glucanase production was obtained after 48h incubation. Among different nitrogen sources, ammonium nitrate enhanced endo-1,4-β-d-glucanase production. The optimal temperature and pH for enzyme activity were 60°C and 7.0, respectively.