Abstract
BackgroundCampylobacter jejuni (C. jejuni) is a leading cause of acute gastroenteritis in human worldwide. The aim of study was to assess the distribution of sialylated lipooligosaccharide (LOS) classes and capsular genotypes in C. jejuni isolated from Iranian children with gastroenteritis. Furthermore, the level of dnaK gene expression in C. jejuni strains with selected capsular genotypes and LOS classes was intended. Moreover, a comprehensive study of C. jejuni MLST-genotypes and inclusive comparison with peer sequences worldwide was intended.MethodsTwenty clinical C. jejuni strains were isolated from fecal specimens of 280 children aged 0–5 years, suspected of bacterial gastroenteritis, which admitted to 3 children hospitals from May to October, 2018. Distribution of sialylated LOS classes and specific capsular genotypes were investigated in C. jejuni of clinical origin. The expression of dnaK in C. jejuni strains was measured by Real-Time-PCR. MLST-genotyping was performed to investigate the clonal relationship of clinical C. jejuni strains and comparison with inclusive sequences worldwide.ResultsC. jejuni HS23/36c was the predominant genotype (45%), followed by HS2 (20%), and HS19 and HS4 (each 10%). A total of 80% of isolates were assigned to LOS class B and C. Higher expression level of dnaK gene was detected in strains with HS23/36c, HS2 and HS4 capsular genotypes and sialylated LOS classes B or C. MLST analysis showed that isolates were highly diverse and represented 6 different sequence types (STs) and 3 clonal complexes (CCs). CC21 and CC257 were the most dominant CCs (75%) among our C. jejuni strains. No new ST and no common ST with our neighbor countries was detected.ConclusionsThe C. jejuni isolates with LOS class B or C, and capsular genotypes of HS23/36, HS2, HS4 and HS19 were dominant in population under study. The CC21 and CC257 were the largest CCs among our isolates. In overall picture, CC21 and CC353 complexes were the most frequently and widely distributed clonal complexes worldwide, although members of CC353 were not detected in our isolates. This provides a universal picture of movement of dominant Campylobacter strains worldwide.
Highlights
Campylobacter jejuni (C. jejuni) is a leading cause of bacterial foodborne poisoning and acute gastroenteritis in human worldwide [1]
Capsular Polysaccharide (CPS) types HS23/36c were found in 9 isolates (45%) and appeared as dominant CPS among 20 C. jejuni strains
We demonstrated that C. jejuni isolates had the predominant LOS class B and C, and capsular genotypes of HS23/36, HS2, HS4 and HS19, all of which are accused to be associated with the progress of gastroenteritis toward Guillain-Barre syndrome (GBS)
Summary
Campylobacter jejuni (C. jejuni) is a leading cause of bacterial foodborne poisoning and acute gastroenteritis in human worldwide [1]. This bacterium often causes a moderate to severe watery regularly self-limiting gastroenteritis and post-infectious immune disorders such as Guillain-Barre syndrome (GBS) [2]. CPS genotyping was used as a more effective method, since it is not affected by variations in capsular gene expression which is observed in serotyping [2]. The aim of study was to assess the distribution of sialylated lipooligosaccharide (LOS) classes and capsular genotypes in C. jejuni isolated from Iranian children with gastroenteritis. The level of dnaK gene expression in C. jejuni strains with selected capsular genotypes and LOS classes was intended. A comprehensive study of C. jejuni MLST-genotypes and inclusive comparison with peer sequences worldwide was intended
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