High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk

Sima Hassani,Mir-Hassan Moosavy,Abolfazl Hajibemani,Seyed Amin Khatibi,Sahar Nouri Gharajalar,Zahra Barabadi

doi:10.1038/s41598-022-07845-6

Sima Hassani, Mir-Hassan Moosavy + Show 4 more

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https://doi.org/10.1038/s41598-022-07845-6

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This study aimed to investigate the prevalence of foodborne pathogenic bacteria in bovine milk, their antibiogram phenotype, and the carriage of antibiotic resistance genes. Raw bovine milk samples (n = 100) were randomly collected from different suppliers in the northwest of Iran. Antibiotic-resistant patterns and the presence of antibiotic resistance genes were evaluated in the isolates. Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. were isolated from 78%, 47%, 25%, and 21% of samples, respectively. All isolates showed high rates of resistance to amoxicillin, penicillin, and cefalexin. The blaTEM and blaSHV genes were detected in 50.0% and 6.4% of E. coli isolates, respectively. Also, 28.5% and 19.0% of Salmonella isolates were positive for blaTEM and blaSHV. The frequency of mecA and blaZ in S. aureus isolates was 20.0% and 12.0%, respectively. The high prevalence of bovine milk contamination with antimicrobial-resistant species in this study necessitates precise control on antibiotic prescription in veterinary medicine.

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This study aimed to investigate the prevalence of foodborne pathogenic bacteria in bovine milk, their antibiogram phenotype, and the carriage of antibiotic resistance genes
Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, and Escherichia coli are the most common pathogens that can be found in raw milk and dairy products made from raw m ilk[4,7]
This study aimed to evaluate the prevalence of pathogenic foodborne bacteria in raw bovine milk through culture-based techniques, their antibiogram phenotype, and the presence of antibiotic resistance genes among the isolates using multiplex-PCR

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Introduction

This study aimed to investigate the prevalence of foodborne pathogenic bacteria in bovine milk, their antibiogram phenotype, and the carriage of antibiotic resistance genes. Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. were isolated from 78%, 47%, 25%, and 21% of samples, respectively. The blaTEM and blaSHV genes were detected in 50.0% and 6.4% of E. coli isolates, respectively. The high prevalence of bovine milk contamination with antimicrobial-resistant species in this study necessitates precise control on antibiotic prescription in veterinary medicine. Due to the potential presence of pathogens and their toxins, the consumption of raw milk can pose a serious risk of foodborne disease to public h ealth[5–8]. Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, and Escherichia coli are the most common pathogens that can be found in raw milk and dairy products made from raw m ilk[4,7]. It seems important to investigate the antibiotic resistance patterns of pathogenic bacteria and the presence of associated encoding genes as the key elements of antibiotics resistance

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