Abstract
We introduce a high-pressure cell for fluorescence fluctuation spectroscopy. The pressure cell consists of a cylindrical, fused silica microcapillary and a modified pressure plug, which connects the cell to a pressure generating apparatus. The capillary serves both as the body and the optical window of the cell. The pressure cell is mounted onto the stage of an optical microscope. The small diameter of the capillary allows the use of high numerical aperture objectives, which are required for fluorescence fluctuation experiments. The pressure stability of the cell has been tested up to 4 kbar. We performed two-photon excitation experiments on simple dyes as a function of pressure. The diffusion coefficient was determined by fluorescence correlation spectroscopy. We also used the same cell to measure the pressure dependence of fluorescence lifetimes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
