Abstract
Purpose: Evaluate the effect of preincubation of oocytes prior to IVF or ICSI cycles with embryo transfer at blastocyst stage. Methods: Retrospective non randomized study based on secondary analysis of data. Setting: Laboratory of Assisted Reproduction at the Alcivar Hospital. Patients: One hundred-eighteen cycles of IVF and ICSI were analyzed in the present study. The evaluated groups were formed for those patients whose oocytes, after retrieval, were inseminated at 1-3 h (Group I) or 4-6 h (Group II). Results: There was no difference in fertilization rate (83.6% and 78.1%), Day 3 cleavage rate (95.1% and 97.1%), and blastocyst formation (31.1% and 39.1%) for groups I and II respectively. Clinical pregnancy rates (PR: 53.0% vs 22.9%) and implantation rates (IR: 38.1% vs 13.0%) were significantly higher in group II versus group I, respectively (P < 0.05). Conclusions: Preincubation of oocytes before insemination is a factor which raises the PR and IR after the blastocyst transfer.
Highlights
The science of In Vitro Fertilization (IVF) has improved considerably in the last 25 years since the first “test-tube” baby was born
Embryo quality derived from oocytes that were inseminated 1 - 3 h was similar to those that were inseminated at 4 - 6 h (76.0% vs 80.1%)
Complete nuclear and cytoplasmic maturation of oocytes is essential for the activation of oocytes at fertilization and the development of embryos [28]
Summary
The science of In Vitro Fertilization (IVF) has improved considerably in the last 25 years since the first “test-tube” baby was born. Developmental competence, is acquired during folliculogenesis as the oocyte grows and during the period of oocyte maturation. As an oocyte grows and matures, it acquires the abilities to resume and complete meiosis, successfully undergo the fertilization process, and initiate and sustain embryonic development [2]. In the course of acquiring these competences, cytoplasmic changes occur, which may include such cellular processes as mRNA transcription [3,4,5], protein translation [6], ultrastructural changes [7,8] and post-translational modification of proteins [9], all involved in meiotic progression and cell cycle control [10], and other proteins involved in cellular processes critical for developmental success before and after activation of the zygote genome [11,12]
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