Abstract
Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) can catalyze the cleavage of a-1, 4-glycosidic bonds of polygalacturonate by a trans-elimination reaction and generate an unsaturated oligogalacturonates. As the critical enzyme of many environmental friendly processes, alkaline PGL has been widely used in many fields including paper, textile and beverage industries. At present, Bacillus subtilis is an ideal strain for producing PGL, but the yield is too low for industrial production. In this study, the effect of different SD sequences on the production of PGL was comparatively investigated, and the strong SD sequence (AGAGAACAAGGAGGG G) directed efficient PGL secretory expression and increased PGL yield to 264.5 U·mL-1 with a high productivity. As a result, the PGL yield in B. subtilis was effecively increased and laid the solid foundation for PGL industrial production.
Highlights
In recent years, due to vigorous development of the chemical industry, global environment has been seriously polluted
There have been some reports on the production of PGL by B. subtilis, but they are all focused on wild bacterial strain screening and medium optimization[5]
Cell growth was monitored by measuring the optical density (OD) of culture broth using a spectrophotometer at 600 nm after an appropriate dilution
Summary
Due to vigorous development of the chemical industry, global environment has been seriously polluted. Traditional textile wet treatment process includes three steps of desizing, refining and bleaching, which uses water as the medium and reacts under high temperature and high alkali conditions. It destroys the structure of cotton and linen fabrics, consumes a lot of heat energy, and produces a lot of industrial waste water. There have been some reports on the production of PGL by B. subtilis, but they are all focused on wild bacterial strain screening and medium optimization[5]. In order to further increase the yield of PGL, this study explored the most suitable SD sequence for PGL gene expression. By screening the most suitable SD sequence, the yield of PGL was further improved and laid a solid foundation for industrial production of PGL
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