Abstract
Alkaline polygalacturonate lyase (PGL), which was the central enzyme of green environmental enzyme treatment technology, has been widely used in textile, papermaking and beverage production. In the previous work, Bacillus subtilis was used as the expression host, and the PGL gene derived from B. subtilis WSHB04-02 was successfully expressed in the engineered strain WB43CB by regulating of molecular elements such as signal peptide, promoter and SD sequence. In this study, the fermentation medium was optimized from four aspects of carbon source, nitrogen source, liquid volume and inoculum volume, and the extracellular PGL enzyme activity in WB43CB increased from 264.5 U·mL-1 to 461.96 U·mL-1, which laid a solid foundation for the industrial production of PGL.
Highlights
In recent years, due to the importance of environmental issues and the pressure from the public, many countries have enacted environmental legislation, forcing industrial producers to seek environmentally friendly processes to replace traditional chemical processes
Among the many enzyme products used in the textile industry, alkaline pectinase with strong alkali resistance, high temperature resistance, and a wide range of sources, has been widely used in the textile industry, paper industry and food industry[2]
B. subtilisWSHB04-02 was screened by our laboratory, and WB43CB was constructed by this research
Summary
Due to the importance of environmental issues and the pressure from the public, many countries have enacted environmental legislation, forcing industrial producers to seek environmentally friendly processes to replace traditional chemical processes. Along with the rapid development of genetic engineering and protein engineering, biological enzyme preparations have used in many industrial productions replacing traditional chemicals. Among the many enzyme products used in the textile industry, alkaline pectinase with strong alkali resistance, high temperature resistance, and a wide range of sources, has been widely used in the textile industry, paper industry and food industry[2]. Research mainly focus on selection and construction of wild strains producing alkaline pectinase. B. subtilis is considered to be the most suitable strain for PGL production by the features of strong protein secretion system, good safety performance, no obvious codon preference, clear genetic background research and simple fermentation conditions[3-5]
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