High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

Abstract

A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a] pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass spectrometry (MS/MS). Chromatographic separation was performed on a Waters Acquity UPLC BEHC18 column (1.7 microm, 2.1 mm x 50 mm). A 0.2 microm precolumn filter was used to protect the analytical column. Mobile phase Awas acetonitrile containing 0.5% toluene. Mobile phase B was water. Linearity of detection was in the range of 1-100 microg L(-1); LOD of 5 PAHs were lower than 0.1 microg L(-1); LOQ were 0.2 microg L(-1) except for benzo[k]fluoranthene. The LOD and the LOQ of benzo[k]fluoranthene were respectively 0.1 microg L(-1) and 0.8 microg L(-1). Wastewater samples collected from two wastewater treatment plants were determined using this method respectively. Recovery of all compounds varied from 67.8 +/- 10.6% to 113.2 +/- 7.2%. In comparison with the existing methods, this rapid method saves time and solvent and improves instrument sample throughput by 2-5 fold.