Abstract
The HPLC method, which has been developed in our previous reports, was examined whether it can be applied to simultaneous, efficient separation and identification of insulin from different species (equine, porcine, bovine and ovine). All of them could be satisfactorily chromatographed under isocratic elution system by using Nucleosil CN as a stationary phase and buffer solution (H3PO4-KH2PO4) as mobile phase.
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