Abstract
Recently several papers have shown that proteins can be separated by using gradient elution method in reversed phase partition high performance liquid chromatography (HPLC) system, but there has been no report on the use of isocratic elution method. The HPLC method, which has been developed in previous report, was examined whether it can be applied to achieve efficient separation and identification of proteins. Under isocratic elution system by use of Nucleosil CN as stationary phase and sodium octanesulfonate as ion-pair reagent in mobile phase, we studied simultaneous spearation of five kinds of proteins ; insulin, ribonuclease, myoglobin, lysozyme chloride and cytochrome C. Under optimal conditions all of the proteins tested could be satisfactorily chromatographed.
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