High-performance liquid chromatography of disaccharides on amine-bonded silica columns

Abstract

Twenty different disaccharides (cellobiose, gentiobiose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, leucrose, maltose, maltulose, melibiose, nigerose, palatinose, sophorose, sucrose, α,α-trehalose, α,β-trehalose, β,β-trehalose, turanose, and xylobiose) were subjected to high-performance liquid chromatography on pre-packed amine-bonded silica columns, using acetonitrile—water eluents. Glucopyranosyl-glucoses had increasing retention times in the order of linkage (1→3), (1→4), (1→2) and (1→1), and (1→6). Replacement of one of the glucosyl residues by galactose led to longer retention times, while substitution by a fructosyl residue yielded shorter ones. Forced assumption of the furanose ring form by the fructosyl residue, as in sucrose and palatinose, gave greatly reduced retentions.