Abstract
Solanum glycoalkaloids are gaining increased scientific attention due to their bioactive potential in the defense of plants against pests and pathogens. The comprehensive glycoalkaloid profiling from the leaves, stems, and roots of seven underexploited Solanum species (S. caripense, S. melanocerasum, S. muricatum, S. nigrum, S. quitoense, S. retroflexum, and S. sisymbriifolium) was conducted using high-performance liquid chromatography–time-of-flight mass spectrometry. A total of 51 glycoalkaloids were shared among the studied Solanum species, with concentrations ranging from 7 to 5.63 × 105 ng g−1. Based on the glycoalkaloid composition, plants were separated into two clusters, Cluster 1 (S. melanocerasum, S. nigrum, and S. retroflexum) and Cluster 2 (S. caripense, S. muricatum, S. quitoense, and S. sisymbriifolium). The inhibition activity of glycoalkaloid extracts on acetylcholinesterase showed a half-maximal inhibitory concentration (IC50), ranging from 0.4 (S. nigrum stems) to 344.9 µg mL−1 (S. sisymbriifolium leaves), that was not directly correlated to the total glycoalkaloid contents. This suggests that the composition of glycoalkaloids in the plant extract, rather than the total concentration, is a driver of biological activity. The study provides a framework for the bioprospecting of underexploited Solanum species for exploring bioactive glycoalkaloids and other compounds with potential pesticidal activities for the development of green bioformulation. This is the first comprehensive report on the glycoalkaloid profiles of S. retroflexum.
Highlights
The plants of the Solanaceae family contain a class of biologically active compounds called glycoalkaloids, which represent a wide class of chemical compounds composed of a C27 cholestane skeleton, to which a carbohydrate moiety of one to five monosaccharides is attached [1,2,3,4,5,6,7,8,9]
The presented study provides a framework for creating data sets for further biologically active compound identification and analysis. Using this framework we were able to demonstrate that the composition of glycoalkaloids in plant extracts rather than the total concentration is a driver of inhibitory activity
The S. sisymbriifolium leaf extract with the highest AChE inhibition had a unique glycoalkaloid, tentatively identified as malonyl solanandaine, which was not identified in the other Solanum species used in this study
Summary
The plants of the Solanaceae family contain a class of biologically active compounds called glycoalkaloids, which represent a wide class of chemical compounds composed of a C27 cholestane skeleton, to which a carbohydrate moiety of one to five monosaccharides is attached [1,2,3,4,5,6,7,8,9]. Due to their pesticidal properties, glycoalkaloids have the potential to function well in the field of plant protection. These underexploited plants contain many glycoalkaloids that can potentially have desirable pesticidal properties
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