Abstract
Solanum sisymbriifolium also known as litchi tomato or sticky nightshade is one of the promising feedstocks for the production of nematicides as it was demonstrated to be efficient for the control of a wide range of pests including major potato pests, Globodera rostochiensis and Globodera pallida. While pesticidal effects are often attributed to a biologically active group of compounds called glycoalkaloids, the specific chemistry of the plant is still poorly understood. The objective of this study was to optimize the high-performance liquid chromatography–mass spectrometry analysis and extraction procedure for glycoalkaloid chemical group to get a better insight into glycoalkaloid composition. The results showed that 19 compounds can be identified as glycoalkaloids with five major glycoalkaloids representing 95% of total composition and with solamargine being the most abundant compound. Structure elucidation based on acid hydrolysis and mass spectrometry spectral analysis suggested that all five major glycoalkaloids belong to solasodine type of glycoalkaloids with different methylation functionalities. Extraction of these compounds from plant material was achieved with aqueous methanol solution.
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