Abstract
High-performance size-exclusion chromatography was used to quantitate the amount of glucoamylase (GA) immobilized on various membrane materials. The amount of GA immobilized ranged from 0.39 to 1.90 g/ft 2 of membrane. A Dextro-Pak C 18 plastic cartridge in a radial compression module was used to evaluate the reaction products of the immobilized GA, using 1.0% dextrin as a substrate. The low-molecular-weight oligo-saccharides (degree of polymerization 1–10) in 24.0 and 1.0% dextrin and in the membrane permeates were identified and quantitated in 25 min. High-performance liquid chromatography analyses were demonstrated to be easier and more sensitive than conventional protein assays and colorimetric sugar analyses.
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