Abstract
The reaction between iodine and azide ions induced by benzylthiouracil was applied as a post-column detection system for determination of benzylthiouracil in urine. Neither extraction, nor preconcentration of the sample are necessary. The reproducibility, linearity, and recovery were evaluated under the optimum conditions. The benzylthiouracil standards added to normal urine show that the response of the detector, set at 350 nm (corresponding to unreacted iodine in the post-column iodine-azide reaction), was linear within the concentration range of 0.4-4.5 nmol · mL−1 urine. Recovery and the relative standard deviation values for precision within the calibration range were from 94 to 104% and from 1 to 4.5%, respectively. Lower limits of detection (LLD) and quantitation (LLQ) were 0.3 and 0.4 nmol · mL−1 urine, respectively.
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