Abstract
The performance of electrospray ionization and atmospheric pressure chemical ionisation (APCI) for the analysis of carotenoids was investigated. On our instrument, APCI produced spectra which were readily interpreted, and the positive-ion mode proved applicable to all of the compounds. The separation of nine compounds in under 14 minutes by gradient elution high-performance liquid chromatography (HPLC) has been demonstrated. The performance characteristics of positive-ion HPLC/APCI-mass spectrometry have been investigated and documented for the determination of nine carotenoids. With the exception of β-carotene, the precision of the method expressed as coefficient of variation ranged from 6 to 20%. Limits of detection ranged from 0.1 to 1.4 ng/μL dependent upon the analyte. The method was used to confirm the levels of carotenoids in UK Total Diet Survey samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
