High-performance liquid chromatographic separation of some 2- and 4-hydroxy derivatives of 2'-benzoylbenzoic acid on Florisil and silica gel columns

Abstract

A series of 2'-benzoylbenzoic acid derivatives including some pairs of positional isomers and other related compounds were chromatographed by HPLC using a normal-phase system with polar adsorbents (Florisil and silica) and 2-propanol, 1,4-dioxane and tetrahydrofuran as modifiers of the eluent. The results obtained were compared as log k′ Florisil vs. log k′ silica relationships to ascertain the differences in distribution and chemical character of surface-active centres of the investifated adsorbents and the mechanism of elution in different eluent systems