Abstract
A rapid and selective reversed-phase high-performance liquid chromatographic assay with gradient elution and diode-array detection for diacetylmorphine, morphine, codeine, and their free and glucuronidated metabolites in plasma, was developed. After addition of ethylmorphine as internal standard the plasma samples were extracted using C 18 ODS-2 solid-phase columns with a recovery better than 80%. The limit of quantitation using an injection volume of 2 μl was 25 ng/ml for each compound. The intra- and inter-day precision was better than 5%. The described method cannot only be used for pharmacokinetic studies but also for intoxication cases to monitor a wide range of opiates.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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