Abstract
A rapid, selective, precise reversed-phase liquid chromatographic method has been developed for the determination of a potential memory-enhancing agent (CL 275,838) and its main metabolite (CL 286,527) in plasma and serum. The procedure includes isolation of compounds from proteins precipitated with acetonitrile, subsequent resolution by reversed-phase (Whatman Partisphere C 8) high-performance liquid chromatography and ultraviolet detection. The assay was linear over the range 0.12–1.25 μg/ml of plasma or serum. The detection limit was 0.12 μg/ml, using 0.2 ml of plasma or serum. Intra and inter-day validation studies indicated an acceptable precision and reproducibility of the method within the concentration range investigated, the overall coefficient of variation being less than 10%. The method is currently applied in support of pharmacological and toxicity studies of the compound in rodents.
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