High-performance liquid chromatographic method for simultaneous determination of iloperidone and idebenone in spiked plasma

Abstract

Background: Iloperidone (ILP) is a second-generation atypical antipsychotic agent and idebenone (IDB), a synthetic analogue of coenzyme Q10 is an important cell membrane antioxidant. No validated analytical method for simultaneous determination of ILP and IDB in rat plasma has been reported till date. Objective: To develop and validate a simple reversed phase high performance liquid chromatographymethodforsimultaneousdeterminationofiloperidoneandidebenoneinratplasma. Methods: A liquideliquid extraction method was used for deproteination of plasma samples using methanol as an extraction solvent. Chromatographic separations were done using isocratic conditions. Mobile phase containing acetonitrile and 0.025 M KH2PO4, pH 6 (60:40) at a flow rate of 1 mL/min was utilized for efficient separation. The UV detector was set at 277 nm. Risperidone was used as an internal standard. Results: The limits of detection (LOD) for iloperidone and idebenone were 10 and 20 ng/ml, while the limits of quantification (LOQ) were 30 and 35 ng/ml, respectively. The standard curves for iloperidone and idebenone in plasma were linear over the range of 0.05e20 mg/ ml, with the correlation coefficients of 0.9993 and 0.9985, respectively. All the validation parameters, such as accuracy, intra and inter-day precision were within the required limits. The samples were stable at � 80