Abstract
Hydrogen is an uncontaminated energy carrier as it releases only water and no green house gas is released. Hence, bio-hydrogen production was aimed from Bacillus subtilis , a gram positive bacteria isolated near solid waste dumped place, Namakkal. The isolated strain was confirmed by 16srRNA sequencing. Phylogenetic tree analysis was performed to know the nearest sequences. The isolated strain was allowed to grow in production medium containing beef, yeast extract, peptone, sodium chloride and sugarcane baggase for 16 days. Since, most of the hydrogen producing bacteria grow at broader pH, no pH control was opted during the study. After incubation period, the gas collected was analysed for hydrogen production by gas chromatography. The obtained sequence was submitted to genbank under the accession number KF484756. Phylogenetic analysis revealed the strain as B. subtilis . The hydrogen gas produced by B. subtilis using sugarcane bagasse was in the range of 1.3–3.3 mol H 2 /mol substrate. From this result, we can conclude that the isolated strain is able to produce hydrogen to certain extent and can be improved by gene modification.
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