Abstract
Abstract A reversed phase isocratic high-performance liquid chromatographic method for the analysis of xylitole and hexosaminitols present in the reduced terminal of glyco-saminoglycans is described. The glycosaminoglycans are covalently bound to protein forming proteoglycans via xylose, galactosamine or glucosamine. Alkaline treatment of the proteoglycan in the presence of NaBH4 releases the glycosaminoglycan chains which contain in their reduced terminal xylitole, galactosaminitole or glucosaminitole. The obtained glycosaminoglycans are depolymerized with trifluoroacetic acid and the liberated neutral monosaccharides and xylitole are separated from the hexosa-mines and hexosaminitols on a DOWEX 50-X8 microcolumn. The obtained neutral monosaccharides are reduced to alditols by NaBH4 and separated from xylitole as per-O-benzoylated derivatives on Supelcosil LC-18, using 70 % acetonitrile as eluent, at a flow rate of 1.0 ml/min. Per-O-bcnzoylated derivatives of hexosaminitols are separated from the hexosam...
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
