Abstract
A high-performance liquid chromatographic method was developed for the assay of ofloxacin in serum. The serum samples were filtered through a Molcut II membrane filter after partial deproteinization in order to remove proteins. The filtrate was preseparated on a reversed-phase precolumn and introduced onto a reversed-phase analytical column by applying column-switching techniques. Ofloxacin and nalidixic acid as internal standards were detected by absorbance measurements at 300nm. Using this system the concentration of ofloxacin could be determined at a limit of the order of 20ng/ml, with a linear dynamic range of 80-4000ng/ml. This procedure was applied to pharmacokinetic studies of healthy volunteers treated with ofloxacin.
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