Abstract
Methamphetamine in the human urine wasdirectly determined by high performance liquid chromatography (HPLC) by using a polyvinylalcohlic column and alkali mobile phase. The detailed conditions were as follows : column : Asahipak GS-320M 7.6 mm i.d.×100 mm, column temp. : 40°C, mobile phase : 80% {0.02 M NaHCO3+0.02 M Na2CO3 (8 : 2)} +20% CH3CN, flow rate : 1.0 ml/min, detector : UV photometer (207 nm). The urine was directly injected into HPLC. Methamphetamine was eluted by adsorption chromatography, and separated from endogenous urine constituents. Determination of methamphetamine was carried out successfully by using the calibration curve by peak high (1-10μg/ml). The recovery of methamphetamine added to the human urine (5μg/ml) was 92.0±1.0%.
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