Abstract
Effects of storage conditions on the mutagenicity of human (2 smokers and 3 nonsmokers) urine were examined. Aliquots of urine from each male volunteer were stored at -80, 3, 25 and 40°C for various time points (max. 4 or 8 weeks). All extracts were obtained by the use of the Sep-pak tC18/methanol method. At the end of maximum time points, all extracts were assayed by the pre-incubation method with Salmonella typhimurium YG1024. Some statistical changes of mutagenicity were observed under the storage conditions with 25 and/or 40°C for 1 or 2 weeks by the analysis of variance techniques. However, no statistical changes were observed under -80 and 3°C at least up to 4 weeks (significance level : 0.01). It was suggested that human urine samples for the mutagenicity assay may be practically stored at 3°C at least for 4 weeks.
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