Abstract
Iophenoxic acid (IPA), a marker used to investigate the feeding behaviour of bait-consuming animals has previously been indirectly determined by measuring protein-bound iodine levels in serum or plasma. For the first time a method is reported for the direct determination of IPA in biological fluids. IPA was determined in de-proteinized serum by high-performance liquid chromatography (HPLC) on a C 18 column with a mobile phase of acetonitrile—water. Isocratic and gradient systems are described with limits of detection of 0.2 μg/ml (isocratic) and 0.05 μg/ml (gradient). Recoveries from fox serum were 85% at 0.5 μg/ml, 95% at 5 μg/ml and 91% at 50 μg/ml.
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