Abstract
Abstract A rapid, sensitive, reproducible method that uses a strong anion exchange column and high performance liquid chromatography is described for determining intermediates/side reaction products in FD&C Yellow No. 5. With this method, 3 intermediates (sulfanilic acid, pyrazolone-T, and phenylhydrazinep- sulfonic acid) and 2 side reaction products (ethyl ester of pyrazolone-T and 4,4′-(diazoamino)dibenzenesulfonic acid) are eluted in a reproducible pattern by increasing the ionic strength of a buffered mobile phase. The intermediates sulfanilic acid and pyrazolone- T and the 2 side reaction products are quantitated; the intermediate phenylhydrazine-p-sulf onic acid is detected but not quantitated. Average recoveries for intermediates/side reaction products quantitatively determined in FD&C Yellow No. 5 samples were 99-104%. The amounts of intermediates/ side reaction products recovered were 0.005-0.18% by weight of the total sample.
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