Abstract
Abstract A reverse-phase ion-pair liquid chromatographic method was applied to the measurement of uncombined intermediates and side-reaction products in Tartrazine (FD&C Yellow No. 5). These compounds, sulfanilic acid, phenylhydrazine-p-sulfonic acid, pyrazolone-T, methyl and ethyl esters of pyrazolone-T, and 4,4'-(diazoamino)-dibenzenesulfonic acid, form strong ion pairs with the tetrabutylammonium (TBA) counterion, which are separated on an RP-18 column with a methanol-water solvent mixture under acidic conditions. Baseline resolution is attained with a 0.03 mol/L TBA concentration and a pH of 2.5. With a linear step flow program and 40°C column temperature, elution time is reduced to 20 min with no equilibration between individual runs. Performance data for quantitative measurement are given in terms of calibration curve linearity (r between 0.98 and 1.00 for all compounds studied) and method detection limit (from 0.004 to 0.022% as % per weight of Tartrazine).
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