Abstract
Abstract A high-performance liquid chromatographic assay was developed for the analysis of the beta-adrenergic blocking agent indenolol as a bulk material and in tablet dosage form (Pulsan(R)). Chromatography was done on a reversed-phase system using a Micropak CN-10 column. The mobile phase consisted of 66.7% v/v methanol and 33.3% v/v aqueous acetate buffer of pH 7.0. Detection was performed at the peak maximum of 250 nm utilizing a variable U.V. wavelength detector. Authentic indenolol hydrochloride was employed to establish the calibration curve; and linearity between peak area and concentration proved to be in the concentration range of 2.0 μg/ml to 20 μg/ml. The purity of authentic indenolol hydrochloride powder was checked by non-aqueous potentiometric titration using standard acetous perchloric acid in presence of mercuric acetate.
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