Abstract
A simple and rapid method for the quantitative analysis of carnitine hydrochloride (CH) in the herbal liquores used as gastrointestinal medicines was studied. The herbal liquores were passed through a Mega Bond Elut C18 and a Sep Pak alumina B cartridge for clean-up, and the passing solution was subjected to reversed-phase ion-pair high performance liquid chromatography on an octadecylsilylated silica-gel column (TSKgel ODS-80TM) with spectorophotometric detection at 210 nm. Standard curves were linear in the range from 0.2 to 1.0 mg/ml. The recoveries of CH added to twenty herbal liquores were obtained from 98% to 101%. The present method was appliciable to the determination of CH in the herbal liquores without interference of other principles belonging to Chinese medicinal herbs.
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