Abstract
A method for 4-methylimidazole (4MI) extraction and quantitation in body fluids and forage samples was developed. The procedures involve ion-pair extraction of the compound with the quantitation done by ion-pair liquid chromatography. The results indicate that this high-performance liquid chromatographic method is sensitive, reproducible and more rapid than others that have been previously used. The mean recovery of 4MI from plasma and tall fescue ( Festuca arundinacea) hay samples were above 95 and 85%, respectively. The versatility of the procedure makes it suitable for the determination of 4MI in body fluids and in forage samples.
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